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. Author manuscript; available in PMC: 2011 Jul 14.
Published in final edited form as: Structure. 2010 Jul 14;18(7):787–797. doi: 10.1016/j.str.2010.04.006

Figure 6. Soak of apo-crystal with SAM.

Figure 6

(a) 2Fo-Fc map of the unliganded aptamer with the final model (PDB 3IQP) superimposed. (b) 2Fo-Fc map of the SAM-soaked apo-crystal in which the free state RNA was used as the model. Note extra density corresponding to the position of A46 (1) and SAM (2) in the bound structure. (c) 2Fo-Fc map of the same SAM soaked crystal in which the bound RNA (3IQR) was used as the model. Note that A46 is now well defined by density, and there still is unaccounted for density in the SAM binding pocket. (d) 2Fo-Fc map of the same crystal in which the bound RNA and SAM was used as the search model. The density around SAM is well defined indicating the presence of ligand.