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. Author manuscript; available in PMC: 2010 Aug 9.
Published in final edited form as: Arthritis Rheum. 2009 May;60(5):1282–1293. doi: 10.1002/art.24488

Figure 5.

Figure 5

Synergistic effect of EGCG and TNFα in inducing RA synovial fibroblast cytotoxicity. A, RA synovial fibroblasts were incubated with EGCG (10 and 50 μM) alone or with a combination of EGCG and TNFα (20 ng/ml) for 72 hours in RPMI 1640 plus 1% fetal bovine serum (FBS). Fibroblasts were stained with hematoxylin and eosin, and photomicrographs were obtained. Results are representative of experiments conducted using RA synovial fibroblasts from 3 different donors (original magnification × 200). B, Cell survival was determined using quantitative cell enumeration assays in RA synovial fibroblasts treated with EGCG (10 and 50 μM) alone, TNFα (20 ng/ml) alone, or a combination of EGCG and TNFα for 72 hours in RPMI 1640 plus 1% FBS. Bars show the mean and SEM from 3 independent experiments using RA synovial fibroblasts from 3 different donors, conducted in 4 wells per condition in each experiment. See Figure 1 for other definitions.