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. Author manuscript; available in PMC: 2010 Aug 9.
Published in final edited form as: Dev Cell. 2008 Mar;14(3):437–445. doi: 10.1016/j.devcel.2008.01.002

Figure 1. Alleles of myf5 and myf6 Cre Drivers.

Figure 1

(A) myf5 and myf6 genes are linked on chromosome 10. The 3′UTR of myf6 (in myf6-ICN)or myf5 (in myf5-ICN) harbors an encephalomyocarditis Internal Ribosomal Entry Site (IRES) that is linked to the Cre coding sequence and is followed by the Neomycin (neo)-resistance selection cassette with MC1 promoter. The selection cassette with MC1 promoter is flanked by two FRT sequences.

(B and C) Breeding with FLP-e mice leads to recombination between FRT sites, generating the “NN” alleles.

(D–G) Semiquantitative RT-PCR on total RNA from E12.5 embryos shows (D) reduced expression of myf6 in myf6-ICN+/− mice (star) but (E) normal expression in myf6-NN+/− mice compared to wild-type. Similarly, myf5 expression is (F) reduced in myf5-ICN+/− mice (star) but (G) normal in myf5-NN+/− mice compared to wild-type.

(H and I) β-gal staining revealed a reduced myf6 lineage in E15.5 (H) myf6-ICN/R-LZ embryos compared to (I) myf6-NN/R-LZ embryos.

(J and K) Similarly, the myf5 lineage in E11.5 (J) myf5-ICN/R-LZ embryos is reduced compared to (K) myf5-NN/R-LZ embryos.

(L) A control wild-type E11.5 embryo shows an absence of any β-gal staining.