Fig. 5. Induction of apoptosis by N- and C-terminal truncation mutants of NDG1 in 293T cells. (A) A schematic diagram showing the regions of NDG1 that were truncated to generate a panel of NDG1 variants. An initiating Met codon was included at the N-terminus of the del 31 and 70 mutants. (B) Expression levels of the truncation mutants in transiently transfected 293T cells were analyzed by immunoblotting with anti-FLAG antibodies. (C) Induction of apoptosis by the truncation and full-length variants of NDG1. Apoptosis was scored by obtaining a ratio of punctated DAPI stained cells and the total number of GFP positive cells. For each time point, >200 cells were scored.