Fig. 1. Expression and localization of truncated sortilin. Myc-tagged full-length and a truncated sortilin construct that lacked its cytosolic domain (A) (residues 540–600) were in vitro translated and run on a 12.5% SDS–polyacrylamide gel. The full-length construct ran to the standard 95 kDa (B, lane 1) while the truncated construct was slightly smaller (B, lane 2). The truncated construct was transfected into COS-7 cells and localized in the perinuclear region (C, anti-myc). This staining pattern was merged to the Golgi staining with anti-Golgin, indicating that the truncated sortilin was being retained in the Golgi apparatus (merged). The inset shows the immunofluorescent staining of full-length sortilin. Scale bar = 10 µm.