Fig. 3. Effect of dominant-negative sortilin lacking its cytoplasmic domain in COS-7 cells immunostained with anti-G_M2AP antibody. COS-7 cells were immunostained with anti-G_M2AP antibody following a transfection with truncated sortilin (A). COS-7 cells were also stained for the truncated sortilin construct (B). The merged image shows G_M2AP in a perinuclear staining pattern in the same compartment as the truncated sortilin construct (C). Immunostaining for endogenous G_M2AP demonstrates its normal distribution in non-transfected COS-7 cells (D). Scale bar = 20 µm.