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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: J Immunol. 2010 Jun 11;185(2):1205–1214. doi: 10.4049/jimmunol.1000039

Fig. 3. Eosinophils recruited to the airways in response to allergen challenge express Gal-3.

Fig. 3

BALF cells from OVA-challenged WT mice were dual stained with rat mAb against murine MBP and rabbit anti-human Gal-3 antibodies followed by FITC-conjugated goat anti-rat IgG and Rhodamine Red-X-conjugated goat anti-rabbit IgG to detect the bound primary antibodies. Cells were evaluated by confocal microscopy (magnification of ×600). Arrow heads indicate cells in a representative field that were positive for MBP as well as Gal-3 (A). BALF cells (non-permeabilized) from WT OVA-challenged mice were analyzed for surface Gal-3 expression by flow cytometry after treatment with Fc blocking antibody using total IgG purified from rabbit anti-Gal-3 serum as the primary antibody. Rabbit IgG was used as a control. PE-conjugated goat anti-rabbit IgG was used as the secondary antibody. Side-scatter profiles for control IgG (left) and anti-Gal-3 antibody (right) are shown (B).

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