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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: J Immunol. 2010 Jun 11;185(2):1205–1214. doi: 10.4049/jimmunol.1000039

Fig. 5. Allergen-challenged Gal-3 KO mice exhibit decreased eotaxin-1 levels.

Fig. 5

Eotaxin-1 levels in the BALF of control and OVA-challenged WT and Gal-3 KO mice (n= 7–9/group) were measured by ELISA. Concentration of eotaxin-1 in the BALF was determined against a generated standard curve (range 7.8 to 250 pg/ml) and expressed as pg/ml of BALF (mean ± SE) (A). Eotaxin-1 expression in lung tissue of control and chronic OVA-challenged WT and Gal-3 KO mice was evaluated by immunostaining using rat mAb against murine eotaxin-1. Representative images from each group at a magnification of ×100 are shown (B). Expression of eotaxin-1 mRNA level in lung tissue of control and OVA-challenged WT and Gal-3 KO mice (n = 7/group) was detected by qPCR. Results were expressed as mean ± SE of fold change (2−ΔΔCT) in eotaxin-1 expression after subtraction of internal β-actin control (C). *p < 0.05 compared with WT OVA mice.

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