Fig. 7. TGF-β1 expression is decreased in allergen-challenged Gal-3 KO mice.
TGF-β1 expression in lung tissue of control and OVA-challenged WT mice and Gal-3 KO mice (n = 6/group) was detected by immunohistochemistry with polyclonal antibodies against TGF-β1. Representative images from each group at a magnification of ×100 are shown (A). Level of expression of mature TGF-β1 (25 kD) was quantitated from densitometric analysis of Western blots of lung tissue lysates from control and OVA-challenged WT and Gal-3 KO mice (n = 4/group) after normalizing for β-actin expression and expressed as mean ± SE (B). *p < 0.05 when compared with WT OVA mice.