Fig. 9. Bone marrow leukocytes from Gal-3 KO mice exhibit decreased rolling on endothelial adhesion molecules.

Bone marrow leukocytes (non-permeabilized) from WT and Gal-3 KO mice were analyzed for surface Gal-3 expression by flow cytometry using total IgG purified from rabbit anti-Gal-3 serum (10 µg/ml) as the primary antibody after treatment with Fc blocking antibody. PE-conjugated goat anti-rabbit IgG (10 µg/ml) was used as the secondary antibody. Side-scatter profiles for bone marrow leukocytes from WT (left) and Gal-3 KO (right) mice are shown (A). Single cell suspensions of bone marrow leukocytes from WT and Gal-3 KO mice were infused into a flow chamber containing cover-slips coated with rmGal-3 or rmVCAM-1 at a flow rate of 1 ml/minute for 5 minutes. ICAM-1 was used as a negative control. In some experiments, bone marrow leukocytes were pre-incubated with lactose or maltose (as a control) at a concentration of 3 mM before infusion. Results were expressed as the number of rolling cells per minute (mean ± SE) (B). *p <0.01 versus rolling of untreated WT BM cells on rmGal-3; **p <0.01 versus rolling of untreated WT BM cells on rmVCAM-1; ^#p <0.01 versus rolling of untreated WT BM cells on rmGal-3; ^## p <0.05 versus rolling of untreated WT BM cells on rmVCAM-1.