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. Author manuscript; available in PMC: 2010 Aug 9.
Published in final edited form as: Am J Physiol Renal Physiol. 2007 Mar 6;293(2):F470–F475. doi: 10.1152/ajprenal.00067.2007

Fig. 3.

Fig. 3

Immunohistochemistry for c-Kit and endothelial nitric oxide synthase (eNOS) in the adventitia of the venous limb of the AV fistula. A: c-Kit-positive immunoreactivity (green) in adventitial microvessels, exhibiting lumina, some of which are marked by asterisks. B: eNOS-positive immunoreactivity (red) in the same adventitial microvessels. C: merged image showing combined immunostaining (yellow) in all 4 adventitial microvessels, as indicated by white arrows. Bottom: Western blot analysis demonstrating expression of eNOS in the venous limb of the AV fistula and sham-operated rats; equivalency of loading of the gel was assessed by immunostaining for β-actin.