Fig. 4. Quantitative analysis of telomere movements. (A) A plot of MSD against Δt for telomeres stained with either cy3-PNA or CFP–TRF2. Data represent average values of 100 telomeres (derived from five cells) for cy3-PNA and 25 telomeres for CFP–TRF2 (derived from two cells). Both plots show an indicative for constraint diffusion and reach a plateau at ∼0.2 µm²/s. (B) MSD plot of three populations of cy3-PNA labeled telomeres demonstrating different movement behavior. The squares represent the most constrained population, which is also shown in (A). The triangles represent a subpopulation of telomeres (10% of all telomeres) that show faster movement over larger distances but that still reach a plateau. The diamonds represent a fast-moving telomere. By plotting MSD/Δt, proportional to the diffusion coefficent, as a function of Δt (Vazquez et al., 2001), it becomes clear that the diffusion coefficient decreases with increasing time intervals for the two slowest populations (C and D), consistent with constrained movement, but not for the fast telomere for the time period analyzed (D).