Fig. 2. In situ hybridization of the section of the metatarsal bones from a 5-week-old male wild-type mouse using digoxigenin-labeled mouse bimL (B), procollagen type IA (C) and type II A (D) riboprobes, and TRAP enzymatic staining (A). The labeling was detected by anti-digoxigenin antibody and Alexa 488-labeled anti-rabbit IgG antibody. Note the co-localization of bim transcripts with TRAP staining (OCs) (A and B). No positive bimL staining was co-localized with procollagen type IA staining (osteoblasts) or type IIB staining (chondrocytes). X-gal staining of the tibia from 5-week-old transgenic mice in which lacZ gene was introduced into the bim locus by homologous recombination also showed the clear positive staining in OCs but not in chondrocytes or osteoblasts (E).