Fig. 6. Effect of Csx1 and Spc1 on atf1⁺ mRNA turnover. (A) In vivo localization of Csx1–GFP expressed at endogenous levels, after treatment with 1 mM H₂O₂ (0–30 min). (B) Northern blot of atf1 and pcr1⁺ mRNAs from cells treated with RNA polymerase inhibitor 1,10-phenanthroline (300 µg/ml) with or without exposure to 1 mM H₂O₂ or 0.8 M KCl. (C) Quantification of atf1 and pcr1⁺ mRNA levels from (B) (only oxidative stress is represented).