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. Author manuscript; available in PMC: 2010 Aug 9.
Published in final edited form as: J Cell Biochem. 2009 May 1;107(1):144–154. doi: 10.1002/jcb.22108

Figure 4. Regulation of glycosaminoglycan modifying enzymes gene expression by Runx2 in osteoprogenitors.

Figure 4

Fold changes in mRNA expression of genes encoding glycosaminoglycan (GAG) modifying enzymes including different heparan sulfate and chondroitin sulfate sulfotransferases species upon expression of Runx2 (A), or the same data as in Panel B plotted in a manner that emphasizes relative changes in the expression ratios of enzymes involved in N- versus O-linked modifications of GAGs (B). Similar to Fig. 3, relative mRNA levels were normalized to 18S ribosomal RNA levels and plotted as fold change upon exogenous Runx2 expression in Runx2 null cells. Error bars represent SE between three independent experiments. Statistical significance of differences was determined by Student’s T-test. Values with P < 0.05 are indicated by asterisks and values with P < 0.01 have two asterisks. Error bars represented Standard error of mean (SE) between three independent experiments. Data on expression of two glycosyltransferases (Ext 1 and Ext2) that did not fit the scale of the graph in Fig. 4A were included in Fig. 3B.