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. Author manuscript; available in PMC: 2010 Aug 9.
Published in final edited form as: J Med Primatol. 2008 Dec;37(Suppl 2):33–45. doi: 10.1111/j.1600-0684.2008.00323.x

Figure 3. Comparison of active caspase-3, cleaved PARP, and TUNEL for detection of T lymphocyte apoptosis in SIV-infected rhesus macaques.

Figure 3

(A) Representative flow plots of CD3+ T lymphocytes stained for TUNEL-positive cells (on the left), active caspase-3-positive cells (in the middle) and cleaved PARP-positive cells, including PARP and caspase-3 colocalization (on the right). Data are shown for pre-SIV and two weeks post SIV infection time-points in one rhesus macaque. (B) Kinetics of active caspase-3-, TUNEL- and cleaved PARP-positive T lymphocytes following SIVmac239 infection. Data on two rhesus macaques, 83.05 (on the left) and 85.05 (on the right) are shown. (C) Correlation between active caspase-3-positive and TUNEL-positive T lymphocytes (on the left) and between active caspase-3-positive and cleaved PARP-positive T lymphocytes (on the right). Data on peripheral blood and lymph node of two SIVmac239-infected rhesus macaques shown. Correlation analysis was performed with the Pearson test. “p.i.” – post-infection