Fig. 1. YaeL PDZ region and complementation abilities of mutant forms of YaeL. (A) The amino acid sequence of the YaeL PDZ homologous region and the mutational alterations constructed in this study. The entire region of this sequence was deleted in YaeLΔPDZ. (B) Complementation abilities of the PDZ mutants of YaeL. Strain KK31 [yaeL::kan/pKK6 (Para-yaeL)] was transformed with plasmids encoding the indicated forms of YaeL-His₆-Myc under the lac promoter control. They were pKK11 (YaeL⁺-His₆-Myc), pTWV228 (vector), pKK131 (YaeLΔPDZ-His₆-Myc), pKK135 [YaeLΔPDZ(D402N)-His₆-Myc], pKK138 [YaeL(G214Q)-His₆-Myc], pKK139 [YaeL(A234K/A235K)-His₆-Myc], pKK140 [YaeL(G243Q)-His₆-Myc], pKK136 [YaeL(D244K)-His₆-Myc] and pKK141 [YaeL(I246Y)-His₆-Myc]. Cultures in l-arabinose (0.2%) were diluted 10³-fold with 0.9% NaCl solution and 4 µl portions (containing ∼4 × 10³ cells) were spotted on an L agar plate containing 0.2% arabinose (Ara) or 1 mM IPTG. The plates were incubated at 37°C for 14 h.