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. 2010 Sep;51(9):2504–2515. doi: 10.1194/jlr.M001958

Fig. 7.

Fig. 7.

BBR inhibits HFD-induced upregulation of DNA methylation in the MTTP promoter. A: Percentage of DNA methylation was determined from subcloned PCR products in the pGM-T vector (10 clones from each rat, n = 4 per group). Shown is the mean methylation level. B: Methylation degree (%) of each indicated cytosine phosphodiester bond guanine (CpG) site was determined for rats of the three indicated groups, calculated as the ratio of the number of methylated cytosine to the total number of samples. C–D: Negative correlations between the MTTP mRNA expression level and the degree of DNA methylation at −113 (C) and −20 (D) CpG sites in the liver. Values are mean ± SEM. *P < 0.05, **P < 0.01 versus ND; #P < 0.05, ##P < 0.01 versus HFD. BBR, berberine; HFD, high-fat diet; MTTP, microsomal triglyceride transfer protein; ND, normal diet.