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. 2010 Sep;51(9):2516–2526. doi: 10.1194/jlr.M003335

Fig. 3.

Fig. 3.

Hepatic ACL deficiency results in reduced circulating VLDL containing apoB48. Lipoprotein profiles were determined using pooled serum samples (n = 5) by FPLC fractionation from adenovirus-infected mice fed LFD or HFD for 25 days (n = 7–8/group). A and B: TG and cholesterol concentrations were measured in each indicated fraction corresponding to VLDL, IDL/LDL, and HDL, respectively. The relative TG content in VLDL fractions and cholesterol content in HDL are also shown using the areas under curve from the FPLC profiles. C: Mice likewise infected were fed LFD for 28 days (n = 8). Serum samples were pooled (n = 5) and fractionated by FPLC, and the TG concentration in each fraction was measured (left). Equal volumes of the indicated VLDL fractions were subjected to Western immunoblot analysis using the apoB antibody. The bar graph indicates the relative apoB48/apoB100 ratio as quantified by densitometry from the immunoblots. ACL, ATP-citrate lyase; Apo, apolipoprotein; FPLC, fast-protein liquid chromatography; HFD, high-fat diet; LFD, low-fat diet; TG, triglyceride.