Fig. 5.

Impact of ACL knockdown on the expression of genes related to TG metabolism and total hepatic fatty acid composition. Mice at 8 weeks of age were infected with Ad-shLacZ or Ad-shACL and fed LFD or HFD for 25 days. Liver RNAs or total fatty acids were prepared from mice under the fed state. A: The mRNA expression level was determined for each indicated gene involved in acetyl-CoA generation and TG synthesis by real-time quantitative RT-PCR and expressed as relative to that of the Ad-shLacZ-infected control group fed LFD (set as 1.0) with GAPDH used as the internal control. Data are shown as means ± SEM (n = 7–8/group). B: Profiling analysis by GC-MS of liver fatty acid species ranging from 16 to 22 carbons. The relative abundance of each indicated fatty acid is expressed as the percentage of the total fatty acids determined for each sample. Data are shown as means ± SEM (n = 5–6/group). ^*P < 0.05 and ^**P < 0.01 versus Ad-shLacZ-infected mice fed the same diet by ANOVA; ^#P < 0.05, ^##P < 0.01 versus LFD-fed mice infected with the same adenovirus by ANOVA. ACL, ATP-citrate lyase; GC-MS, gas-chromatography mass-spectroscopy; HFD, high-fat diet; LFD, low-fat diet; TG, triglyceride.