Fig. 2.

Dose-response inhibition of GlcCer synthesis following PPMP treatment. A: Isolated parasites were labeled with [³H]palmitic acid for 3 h in presence of solvent (cntl) or PPMP at the indicated concentrations. Alternatively, parasites were pretreated with 10 μM PPMP for 30 min, washed, and labeled with [³H]palmitic acid as described before (Pre). Lipid aliquots corresponding to equal protein amount were separated by 1D-HPTLC using the solvent system B. GlcCer (GC) levels are expressed as percentage of untreated samples (cntl); data are average ± SE (n = 3) of a representative from two experiments done in triplicate. B: Liquid chromatography-mass spectrometry analysis of glucosylceramides extracted from trophozoites treated for 4 h with of 10 μM PPMP or solvent (cntl). Data are average ± SE (n = 3). ^*A significant difference (P < 0.05) compared with the percentage of control treated parasites, two-tailed Student's t-test.