Fig. 1.

Enhancement of phagocytosis by apoA-I and apoA-II. J774 cells (A) were subcultured in a 96-well tray at a density of 4 × 10³cells/well for one day. Mouse peritoneal macrophages (B) were subcultured in a 96-well tray at a density of 1 × 10⁴cells/well for six days. After overnight incubation in the presence or absence of 10 μg/ml of apoA-I or apoA-II, quantitative phagocytosis assay was performed as described. The results were expressed as phagocytic activity relative to the blank background control (without apolipoprotein, 0.02% BSA). Dose-dependent data of apoA-I (μg/ml) are also displayed with a negative control of 10 μM CytD in the presence of apoA-I (10 μg/ml) to inhibit phagocytosis (A and B). The microspheres were precoated with apoA-I or apoA-II prior to incubation with J774 cells as described (C). The results are expressed as the activity relative to each control, activity of the cells for beads precoated and preincubated in 0.02% BSA only as the blank control. The data represent the means ± SD for eight samples. Statistical analysis was performed by Tukey's test to give significance levels of ^***P < 0.001; ^**P < 0.01; ^*P < 0.05 to the control or between the data indicated. The microscopic photo data represent typical results of three independent experiments performed. ApoA, apolipoprotein A; CytD, cytochalasin D.