TABLE 2.
Body weight, food intake, and plasma and hepatic parameters
| Low Fat |
High Fat |
|||
|---|---|---|---|---|
| Ad-Luc | Ad-Elovl5 | Ad-Luc | Ad-Elovl5 | |
| Number of mice/group | 8 | 8 | 8 | 8 |
| Body weight (g) | ||||
| Before injection | 30.9 ± 3.7 | 27.5 ± 1.2 | 42.5 ± 5.0a | 39.2 ± 5.0a |
| After injection | 30.4 ± 3.6 | 27.4 ± 1.6 | 40.9 ± 4.6a | 36.3 ± 4.0a |
| Food intake | ||||
| Gram/day | 3.7 ± 0.8 | 3.3 ± 0.7 | 3.3 ± 1.6 | 2.5 ± 0.7 |
| Kcal/day | 14.1 ± 3.2 | 12.6 ± 2.6 | 17.2 ± 8.5 | 12.8 ± 3.9 |
| Plasma parameters | ||||
| Adiponectin (μg/ml) | 5.2 ± 1.3 | 5.9 ± 0.3 | 6.6 ± 1.0 | 6.2 ± 1.1 |
| NEFA (mEg/ml) | 0.3 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| Cholesterol (mg/dl) | 114.6 ± 42.3 | 173.9 ± 4.9 | 181.3 ± 46.6 | 159.0 ± 0.2 |
| Triglycerides (mg/dl) | 119.6 ± 4.7 | 55.9 ± 12.1b | 91.5 ± 25.0 | 70.7 ± 18.0 |
| β-hydroxybutyrate (mM) | 1.54 ± 0.3 | 1.65 ± 0.4 | 0.6 ± 0.3a | 1.5 ± 0.2 |
| Liver parameters | ||||
| Weight (g) | 1.2 ± 0.1 | 1.5 ± 0.1b | 1.7 ± 0.3a | 1.9 ± 0.3a |
| Percent of body weight | 3.9 ± 0.4 | 5.5 ± 0.3b | 4.2 ± 0.7 | 5.2 ± 0.3b |
| Liver protein (mg protein/mg DNA) | 24.3 ± 6.5 | 18.3 ± 4.2 | 29.0 ± 3.6 | 24.8 ± 4.8 |
| Triglyceride (μg triglyceride/mg protein) | 49.2 ± 25.0 | 66.1 ± 22.6 | 156.9 ± 88.4a | 91.0 ± 17.8 |
| Cholesterol (μg cholesterol/mg protein) | 14.9 ± 2.1 | 13.3 ± 0.7 | 17.4 ± 7.1 | 17.0 ± 3.6 |
| Glycogen (μg glucose/mg protein) | 2.3 ± 2.1 | 4.9 ± 2.2b | 31.9 ± 9.6a | 10.8 ± 7.7b |
Mice were fed a low-fat diet (Research Diets D12450B, 10% calories as fat) or a high-fat diet (Research Diets, D12492, 60% calories as fat) for 12 weeks, ad lib. After 11 weeks on the diet, mice in each group were infected with recombinant adenovirus expressing either luciferase (Ad-Luc) or Elovl5 (Ad-Elovl5). Five days later, mice were fasted overnight and euthanized the next day. Body weights were determined prior to injection on ad lib-fed mice. Final body weight (after injection), plasma, and hepatic parameters were measured in mice fasted overnight (see “Materials and Methods”). Results are presented as mean ± SD. Elovl5, fatty acid elongase-5; Luc, luciferase.
P ≤ 0.05, low fat versus high fat.
P ≤ 0.05 Ad-Luc versus Ad-Elovl5, ANOVA, two-way.