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. 2010 Sep;51(9):2686–2694. doi: 10.1194/jlr.M007906

Fig. 4.

Fig. 4.

Fatty acid composition of phospholipids (PC, PE) and neutral lipids (TG, CE), and cellular free fatty acids. A: FA composition of PC for untreated cells and cells treated with 10 µM CLA or linoleic acid for 4 h, separated by TLC, methylated to generate FAMEs, and analyzed by GC-MS. B: FA composition of PE for untreated cells and cells treated with 10 µM CLA or linoleic acid for 4 h, separated by TLC, methylated to generate FAMEs, and analyzed by GC-MS. C: FA composition of TGs for untreated cells and cells treated with 10 µM CLA or linoleic acid for 4 h, separated by TLC, methylated to generate FAMEs, and analyzed by GC-MS. D: CE species profile of untreated cells and cells treated with 10 µM CLA or linoleic acid for 4 h and analyzed by ESI-MS/MS. E: FFA in untreated cells and cells treated with 10 µM CLA or linoleic acid for 4 h, separated by TLC, methylated to generate FAMEs, and analyzed by GC-MS. Values are mean ± SD of one representative experiment from three, each performed in triplicate. CLA, conjugated linoleic acid; FA, fatty acid; FFA, free fatty acid; FAME, FA methyl ester; PC, phosphatidylcholine; PE, phosphatidylethanolamine.