Fig. 2.

Phenamil is a unique inducer of PPARγ expression in preadipocytes. A: Gene expression profile during differentiation of 3T3-F442A cells treated with GW7845 (20 nM) or phenamil (10 μM). PPARγ target genes aP2 and LPL were induced by GW7845 and phenamil; however, PPARγ expression was acutely induced by phenamil but not by GW7845. B: Induction of C/EBPβ and C/EBPδ by phenamil and standard differentiation cocktail (dexamethasone, IBMX, insulin) in 3T3-F442A cells. Standard differentiation cocktail induced the expression of C/EBPβ and C/EBPδ starting from 2 h treatment in 3T3-F442A cells, whereas phenamil and GW7845 did not alter expression of these two transcription factors. ^*P < 0.05, ^**P < 0.005, ^***P < 0.0005. IBMX, 3-isobutyl-1-methylxanthine; PPARγ, peroxisome proliferator-activated receptor γ.