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. 2010 Sep;51(9):2775–2784. doi: 10.1194/jlr.M008490

Fig. 3.

Fig. 3.

Phenamil and harmine act by different pathways. Phenamil does not modulate the Wnt signaling pathways. A-B: Phenamil effects on β-catenin-driven and Wnt-driven TOP FLASH activity in 293-T cells and endogenous Wnt target gene expression in 3T3-F442A cells. Wnt inhibitor harmine inhibited the activation of Wnt pathway in TOP FLASH (A) and induction of Wnt target genes Axin2 and Id2 (B). Phenamil does not regulate Wnt signaling pathways shown in TOP FLASH experiments (A) and expression of endogenous Wnt target genes (B). C: Phenamil and harmine showed an additive effect on the expression of PPARγ and its target gene aP2. 3T3-F442A cells were treated with harmine (1 μM), phenamil (1 μM), or both for 48 h. *P < 0.05, **P < 0.005; ns, not significant.