Fig. 4.

Identification of genes rapidly induced by phenamil in 3T3-F442A preadipocytes. A: 3T3-F442A cells were treated with either phenamil or GW7845 for 24 h. Comparison of effects by phenamil and GW7845 on ETV1, ETV4, ETV5, and PPARγ gene expression. Realtime PCR analysis confirmed that ETV4, ETV5, and PPARγ were upregulated by phenamil but not by GW7845. The other Pea3 member, ETV1, was not regulated by either phenamil or GW7845. B: Selective regulation of ETV4 and ETV5 by phenamil. ETV4 and ETV5 display a pattern of phenamil-specific induction. 3T3-F442A preadipocytes were treated with phenamil (10 μM) or GW7845 (20 nM) for 48 h at confluence, and mRNA levels were measured by real-time PCR. C: Time-course of regulation of selective genes by phenamil. ETV4 and ETV5 were induced by phenamil as early as 6 h, while PPARγ expression was induced later. Comparison of the effects by phenamil and GW7845 treatment on PPARγ and PPARγ target genes and Id1, trb3 expression are also shown. ^*P < 0.05, ^**P < 0.005, ^***P < 0.0005. ETV1–5, EST variant 1-5; PPARγ, peroxisome proliferator-activated receptor γ.