Skip to main content
. 2010 Sep;51(9):2775–2784. doi: 10.1194/jlr.M008490

Fig. 5.

Fig. 5.

ETV4 stimulates PPARγ expression and adipocyte differentiation in 3T3-L1 cells. A: Transient overexpression of ETV4 induces expression of PPARγ and its target genes in 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were transiently transfected with an ETV4 expression vector or empty vector. Four days later, mRNA was collected, and gene expression was determined by real-time PCR. B-C: Promotion of adipocyte differentiation by stable overexpression of ETV4 in 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were infected with pBabe-based retrovirus harboring the ETV4 gene, and large stable pools were selected with puromycin (2 μg/ml). B: Expression of PPARγ and its target genes from day 0 to day 6. Differentiation of 3T3-L1 cells into adipocytes was induced by DI and GW7845 (GW; 30 nM). C: Oil red O staining of ETV4 overexpressing 3T3-L1 cells. *P < 0.05, **P < 0.005, ***P < 0.0005. ETV4, EST variant 1-4; PPARγ, peroxisome proliferator-activated receptor γ.