Fig. 6.

ETV4 stimulates adipocyte differentiation in 3T3-F442A cells. Overexpression of ETV4 induces expression of PPARγ and adipocyte differentiation in 3T3-F442A cells. A: Oil red O staining of ETV4 overexpressing 3T3-F442A cells. 3T3-F442A preadipocytes were infected with pBabe-based retrovirus harboring the ETV4 gene, and large stable pools were selected with puromycin (2 μg/ml). B: Expression of PPARγ and its target genes on day 6. ^*P < 0.05, ^**P < 0.005, ^***P < 0.0005. ETV4, EST variant 1-4; PPARγ, peroxisome proliferator-activated receptor γ.