Fig. 7.

Knockdown of PPARγ blocks the adipogenic effects of phenamil but does not affect induction of ETV4. Stable 3T3-F442A cells expressing shRNAs against PPARγ were generated. ShRNAs effectively reduce PPARγ mRNA expression (upper left). Expression of ETV4 and ETV5 is not affected by knockdown of PPARγ, demonstrating that ETV4 is upstream of PPARγ (upper middle and right). Expression of downstream target genes of PPARγ, including aP2, adiponectin, and Aqp7, is reduced in PPARγ shRNA-expressing cells, both in the presence and absence of phenamil (bottom). Gene expression was determined by real-time PCR. ^*P < 0.05, ^**P < 0.005, ^***P < 0.0005; ns, not significant. ETV4, EST variant 4; PPARγ, peroxisome proliferator-activated receptor γ.