Figure 3. Sox6 is needed in the stress erythropoietic response to acute anemia.

(A) Kinetics of RBC decay in Sox6^fl/fl and Sox6^fl/+ErGFPCre control and Sox6^fl/flErGFPCre mutant mice over a period of 2 weeks following PHZ treatment. RBCs were labeled with biotin at day –1 and PHZ was injected at the days 0 and 1. The data are presented as the average with standard deviation of values obtained for 3 mice per genotype. (B) Kinetics of hematocrit changes in control and mutant mice following PHZ treatment. Each data is the average with standard deviation of values obtained for 3 mice. Different mice were used at each time point. (C) Mouse survival curves following PHZ treatment. At day 3, each experimental group contained 24 mice. All mice survived in the control groups, whereas 3 mice died at day 4, and 3 at day 5 in the mutant group. (D) Kinetics of sEpo level in the same mice as in panel (B). (E) Kinetics of spleen index in the same mice as in panel (B). (F) Northern blot analysis of Sox6 RNA level in the bone marrow and spleen of Sox6^fl/fl control and Sox6^fl/flErGFPCre mutant mice at various time points following PHZ treatment. Sox6 RNA was detected using a coding-exon-2 probe. Hybridization with an Hbb-b1 probe and staining with ethidium bromide of 18S rRNA are shown as controls for RNA loading. *p<0.05, **p<0.01.