Figure 4. Sox6 ensures quick maturation of erythroblasts and reticulocytes during stress erythropoiesis.

(A) Representative CD71/TER119 FACS profiles of bone marrow and spleen from Sox6^fl/fl control (C) and Sox6^fl/flErGFPCre mutant (M) mice 0, 2, and 4 days after PHZ treatment. Note that a massive increase in CD71⁻/TER119⁺ cells occurred in control and mutant spleens at day 2, reflecting clearance of damaged RBCs. (B) Kinetics of the proportions of erythroblasts versus proerythroblasts in bone marrow and spleen of control and mutant mice from day 0 to day 10 following PHZ treatment. Values were derived from CD71/TER119 FACS profiles and are presented as the average with standard deviation for 3 mice per genotype at each time point. (C) Kinetics of the proportions of uncondensed versus condensed erythroblasts. Values were derived from FSC/TO FACS profiles performed with the same cell populations as in panel (B). (D) Kinetics of the percentage of reticulocytes (CD71^high) present in the blood of control and mutant mice following PHZ treatment. Values are presented as the average with standard deviation for at least 3 mice per genotype at each time point. (E) Representative TO/CD71 FACS profiles of blood cells from control and mutant mice 7 and 10 days after PHZ treatment. *p<0.05, **p<0.01.