Table 1. qRT-PCR Primer Sequences.
Gene Name | Forward Sequence | Reverse Sequence |
Pax7 | 5′-GCTCCGGGGCAGAACTACC-3′ | 5′-GCACGCGGCTAATCGAACTC-3′ |
Myf5 | 5′-ATGGACGTGATGGATGGCTG-3′ | 5′-GCGGCACAAACTCGTCCCCAA-3′ |
MyoD | 5′-GGTCCCTCGCGCCCAAAAGAT-3′ | 5′-CAGTTCTCCCGCCTCTCCTAC-3′ |
Myogenin | 5′-CAGTGCACTGGAGTTCAGCG-3′ | 5′-TTCATCTGGGAAGGCCACAGA-3′ |
MRF4 | 5′-CCCCTTCAGCTACAGACCCAA-3′ | 5′-CCCCCTGGAATGATCGGAAAC-3′ |
GAPDH | 5′-CCACCCATGGCAAATTCC-3′ | 5′-TGGGATTTCCATTGATGACAA-3′ |