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. 2010 Jul 20;7:60. doi: 10.1186/1742-4690-7-60

Figure 2.

Figure 2

Virological and immunological characteristics of patient P. (A) Plasma viral load (diamonds) and CD4 + T-cell counts (triangles) of patient P from March till December 2006. An arrow indicates the probable time of HIV-1 superinfection. A second arrow indicates the start of highly active antiretroviral therapy (HAART) in November 2006. Biological clones were generated from PBMC samples collected in March 2006 (31 st of March) and August 2006, respectively. (B) NJ tree of HIV-1 env-V3 nucleotide fragments obtained from plasma collected from patient P. Separate clusters comprised of strains B3 and B4 are indicated. Env sequences from biological clones are indicated with clone numbers. Symbols in the tree correspond to samples from March 2006 (circles), August 2006 (diamonds) and November 2006 (squares). Reference sequences were from HIV-1 strain HXB2, and subtypes C and D strains, respectively. The scale bar indicates the nucleotide distance between the sequences (as calculated with the Tamura-Nei method [59]).