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Immunohistochemical analysis of proteins identified by nano-LC-Q-TOF-MS/MS in rat brain. Rat brains were removed, fixed with 4% paraformaldehyde, and stained with hematoxylin and eosin (A), anti-GAT 3 antibody (B), anti-GFAP antibody (C), or anti-HSP 70 antibody (D) as described in "Experimental Procedures". The panels show representative photographs from independent experiments from 3 rats. Final magnification × 10.
