Figure 1.
Expression and purification of the DEV pUL38. SDS-PAGE of the expressed peptide in E. coli BL21 (DE3) is shown. M Marker; 1 the total cell proteins uninduced with IPTG; 2 the total cell proteins induced with IPTG; 3 the insoluble fraction after purification with IMAC. The black arrow points to the recombinant pUL38 (approximately 70 kDa).