Figure 7.
Supprerssion of DNA-PKcs up-regulated surface expression of DR5 and down-regulated the expression of c-FLIPs. (A) CEM/VLB100 cells were transfected with a siRNA against DNA-PKcs or scrambled siRNA as a control. After 48 h, the total RNA extracted from transfectant of CEM/VLB100 cells performed RT-PCR analysis to monitor the mRNA levels of DNA-PKcs, DR4/5, c-FLIPL/S , MDR1, and actin as a loading control. (B) The transfectant incubated with an anti-DR4 or -DR5 (1:500), and subsequently labeled with FITC-conjugated secondary antibodies (1:1000) to determine the surface expression of DR4 and DR5. Goat IgG2a was also used as control isotype antibody.