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. 2010 Jul 19;10:193. doi: 10.1186/1471-2180-10-193

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Copyright ©2010 Burgos and Beutin; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Detection of plasmid encoded α-hly genes in E. coli strains. A) Agarose gel (0.7%) with plasmid preparations obtained from E. coli strains. Lanes: D = digoxigenin-labelled molecular weight standard II (Roche); L = molecular weight standard hyperladder I (Bioline); 1 = 374 (phly152); 2 = TPE1313 (pO157); 3 = TPE422 (pEO5); 4 = TPE1030 (pEO5); 5 = 84-3208 (pEO11); 6 = 84-2573 (pEO12); 7 = 84-2195 (pEO9); 8 = 84-2 S (pEO14); 9 = 84-R (pEO13); 10 = CB853 (pEO853); 11 = CB855 (pEO855); 12 = CB857 (pEO857). B) Southern hybridization patterns of plasmid DNA from lanes 1-12 with the α-hlyA specific digoxigenin labelled gene probe generated with primers 10f/r from plasmid pEO5 DNA. The size of hybridizing α-hly plasmids varies from 48 (lane 1) to 157 kb (lane 3).