Table 1.
Relevant properties of strains carrying plasmid and chromosomally encoded α-hly determinants
| PCR products with primers pairsa | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| strain | Serotypeb | Origin, referenced | hly-plasmid (kb) | Plasmid group |
1f/r (678 bp) |
32f/r (671 bp) |
44f/r (685 bp) |
99f/r (650 bp) |
72f/r (695 bp) |
81f/r (773 bp) |
| C4115 | O26:[H11] | human, EPEC [21] | pEO5 (157) | 1 | + | + | + | + | - | - |
| TPE422 | Or:H48 | E. coli K12 (pEO5) [21] | pEO5 (157) | 1 | + | + | + | + | - | - |
| CB9866 | O26:[H11] | cattle, EPEC [21] | pEO5 (157) | 1 | + | + | + | + | - | - |
| CB1027 | O26:[H11] | human, EPEC [21] | pEO5 (157) | 1 | + | + | + | + | - | - |
| CB1030 | O26:[H11] | human, EPEC [21] | pEO5 (157) | 1 | + | + | + | + | - | - |
| IP187 | O26:[H11] | human, EPEC [21] | pEO5 (157) | 1 | + | + | + | + | - | - |
| 84/2195 | Ont:H10 | dog [10] | pEO9 (146) | 1 | + | + | + | + | - | - |
| 84-R | O121:H46 | dog [10] | pEO13 (97) | 1 | + | + | + | + | - | - |
| 374 | Or:H48 | mouse [24] | pHly152 (48) | 2 | + | e) | + | + | - | - |
| 84-3208 | O42:H37 | dog, ETEC[10] | pEO11 (48) | 2 | + | e) | + | + | - | - |
| 84-2573 | O70:NM | dog, ETEC [10] | pEO12 (48) | 2 | + | e) | + | + | - | - |
| CB853 | O138:H14 | pig, STEC [29] | pEO853 (145) | 3 | + | f) | g) | + | - | - |
| CB855 | O138:NM | pig, STEC [29] | pEO855 (140) | 3 | + | f) | g) | + | - | - |
| CB857 | O157:NM | pig, ETEC [42] | pEO857 (97) | 3 | + | f) | g) | + | - | - |
| CB860 | O149:H10 | pig, ETEC [42] | pEO860 (48) | single | + | + | g) | + | - | - |
| 84-2S | O75:H2 | dog [10] | pEO14 (97) | single | - | - | - | - | - | - |
| 536h | O6:K15:H31 | human UPEC [20] | - | n.a | - | - | - | - | + | + |
| 536-14 | O6:K15:H31 | PAI I deletion mutant of 536 [20] | - | n.a | - | - | - | - | + | - |
| 695/83 | O126:H27 | human [19] | - | n.a | - | - | - | - | - | i) |
| J96h | O4:K6 | human UPEC [46] | - | n.a | - | - | - | - | + | j) |
| KK6-16 | E. cloacae | human [26] | - | n.a | k) | - | - | - | - | - |
a) primer pairs and size of the PCR products obtained with strains TPE422 (pEO5) (primers 1f/r, 32f/r and 44f/r) and 536 (primers 81f/r and 72f/r) (see Table 2).
+ = a PCR product of the same size as obtained with strains TPE422 (pEO5) or 536, respectively.
- = no PCR product obtained
PCR products with other sizes than obtained with the reference strains are indicated for their length in bp.
b) O:H serotype, Or = rough LPS, Ont = O-antigen not typable, NM = non motile
c) α = alpha-hemolytic (α-hly genes), e-hly = enterohemolytic (EHEC-hly genes)
d) All strains were from feces if not otherwise indicated, ETEC = enterotoxigenic E. coli, STEC = shigatoxigenic E. coli, UPEC = uropathogenic E. Coli
e) 2000 bp PCR product (2007 bp as calculated from the nucleotide sequence of pEO11 [GenBank FM210249]
f) 2900 bp PCR product (2868 bp as calculated from the nucleotide sequence of pEO860) [GenBank FM210351]
g) 1500 bp PCR products
h) strain carries two α-hly determinants in the chromosome
i) 950 bp PCR product
j) 860 bp PCR product
k) 778 bp PCR product (calculated from nucleotide sequencing of KK6-16 [GenBank FM210352])