Table 3.
Detection of α-hly plasmid specific sequences in porcine STEC strains.
| Size of PCR products with primersa | ||||||
|---|---|---|---|---|---|---|
| Serotype | No. strains | Plasmid group | 1f+1r | 32f + 32r | 44 f + 44r | 99 f + 99r |
| O138:H14b | 4 | 3 | 678 | 2900 | 1500 | 650 |
| O139:H1b | 9 | 3 | 678 | 2900 | 1500 | 650 |
| O139:H1b | 1 | single | 678 | 2000 | 1500 | 650 |
| O141:H4b | 7 | 3 | 678 | 2900 | 1500 | 650 |
| O141:H4b | 3 | 2 | 678 | 2000 | 685 | 650 |
| O2:H32c | 1 | 2 | 678 | 2000 | 685 | 650 |
| O36:H19c | 1 | 2 | 678 | 2000 | 685 | 650 |
a) size of the PCR products (bp) with primers 1f/r, 32f/r, 44f/r and 99f/r as described in Tables 1+ 2. Digestion of PCR products by HinfI resulted in identical patterns between strains corresponding to the size of the PCR products obtained (Table 1)
b) Strains isolated from feces of diseased pigs [29]
c) Strains isolated from pork meat [29]