Figure 4.

Cocktail induced stimulation of inflammatory markers. Prostaglandin E2 was determined in conditioned media from two representative donor derived DC batches as described in M&M (A). PGE2 secretion was highest when imDCs were treated with cocktail 1, 2, 3 and 8 for 24 hours. B) COX2 protein levels were analysed by western blot of lysates from DCs treated with cocktails for 24 h. Actin was used as loading control, and results shown are representative blot from two donors. C) Inhibition of PGE2 secretion induced by cocktail 8 was shown by pre-incubation of imDCs from two different donors for 2 h with the unspecific COX inhibitor indomethazine (indo) at 10 μM or the specific COX-2 inhibitor NS398 at 10 μM. D) The ability of cocktails to induce secretion of IL-23, shown as an average of measurements on 3 different donors. Bars show standard deviation between the three donors.