Table 5.
Yeast 2 hybrid assay shows that TnsC and TnsD can interact in vivo.
| Protein fragment | β-galactosidase activity | |
|---|---|---|
| Fos | Jun | 5.0 |
| E2F1 | RB | 1.0 |
| Empty vector Gal4-AD |
Empty vector Gal4-DB |
0.075 |
| TnsC (1 to 555) wt | TnsD (1 to 507) wt | 1.0 |
| TnsC (1 to 555) wt | TnsD (1 to 436) | 0.075 |
| TnsC (1 to 555) wt | TnsD (1 to 399) | 4.5 |
| TnsC (1 to 555) wt | TnsD (1 to 375) | 5.0 |
| TnsC (1 to 555) wt | TnsD (1 to 350) | 4.5 |
| TnsC (1 to 555) wt | TnsD (1 to 309)a | 4.5 |
| TnsC (1 to 555) wt | TnsD (1 to 293) | 0.075 |
| TnsC (1 to 555) wt | TnsD (8 to 507) | 0.075 |
| TnsC (1 to 555) wt | TnsD (12 to 507) | 0.075 |
| TnsC (1 to 555) wt | TnsD (16 to 507) | 0.075 |
| TnsC (1 to 555) wt | TnsD (22 to 507) | 0.075 |
| TnsC (1 to 555) wt | TnsD (1 to 507) wt | 1.0 |
| TnsC (1 to 480) | TnsD (1 to 507) wt | 1.0 |
| TnsC (1 to 384) | TnsD (1 to 507) wt | 1.0 |
| TnsC (1 to 332) | TnsD (1 to 507) wt | 0.20 |
| TnsC (1 to 293)a | TnsD (1 to 507) wt | 0.20 |
| TnsC (1 to 280) | TnsD (1 to 507) wt | 0.014 |
aDeletion analysis of TnsD and TnsC revealed that this protein-protein interaction activity is contained within TnsC 1 to 293 and TnsD 1 to 309.