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Spectrofluorimetric analysis of UDGs and its complexes with Ugi in the presence of urea: UDGs (wild type and Y66W) or their complexes with Ugi (1 A280/ml) were incubated with different concentrations of urea at room temperature for 2 h and the fluorescence spectra (300–400 nm) were recorded upon excitation at 280 nm. (i) Wild-type UDG; (ii) Y66W UDG; (iii) complex of the wild-type UDG with Ugi; and (iv) complex of the Y66W UDG with Ugi.
