Figure 5.
Native PAGE analysis of secondary structure formation by synthetic 100mers. Synthetic oligonucleotides composed of 25 repeats were annealed in buffer (50 mM Tris–HCl pH 8, 10 mM MgCl2, 20 mM NaCl), and loaded on an 8% native polyacrylamide gel in 1× TBE running buffer. Repeat sequences are shown above the gel. The far right lane contains a 10 bp ladder.