Table 1. Melting temperatures and thermodynamic values for the loss of self-annealed DNA structure.
| Sequence | [µM] | Tm (°C) | ΔHo (kcal/mol) | ΔGo(37°C) (kcal/mol) | Annealed hairpina |
|---|---|---|---|---|---|
| d(GCTG)25 | 1.6 | 68 | 51 | 4.6 | 5′-GCTGGCTGGCTG→ |
| 3′-CGGTCGGTCG← | |||||
| d(CAGG)25 | 1.5 | 61 | 28 | 2.0 | 5′-CAGGCAGGCAGG→ |
| 3′-GGACGGACGGAC← | |||||
| d(CAGC)25 | 1.6 | 55 | 23 | 1.2 | 5′-CAGCCAGCCAGC→ |
| 4 | 52 | 19 | 0.9 | 3′-ACCGACCGACCG← | |
| d(ACTG)25 | 0.67 | 45 | 57 | 1.4 | 5′-ACTGACTGACTG→ |
| 3′-AGTCAGTCAGTC← | |||||
| d(CAGA)25 | 1.4 | 37 | 26 | 0 | 5′-CAGACAGACAGA→ |
| 3′-GACAGACAGAC← | |||||
| d(CCTG)25 | 3.1 | ≤35b | 15b | –0.1b | 5′-CCTGCCTGCCTG→ |
| 3′-GTCCGTCCGTCC← | |||||
| d(TCTG)25 | 1.6 | 31 | 28 | –0.6 | 5′-TCTGTCTGTCTG→ |
| 2.2 | 27 ± 1, n = 2 | 35 ± 1 | –1.2 ± 0.2 | 3′-TGTCTGTCTGTC← | |
| 3.2 | 31 | 30 | –0.6 | ||
| d(CAGT)25 | 1.6 | 24 | 39 | –1.7 | 5′-CAGTCAGTCAGT→ |
| 3′-GACTGACTGAC← |
aWatson–Crick base pairs are shown in bold.
bEstimated.
See Materials and Methods for buffer conditions.