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. Author manuscript; available in PMC: 2011 Aug 6.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jul 16;398(4):719–722. doi: 10.1016/j.bbrc.2010.07.007

Fig. 2.

Fig. 2

EGF-induced PLC-γ1 activation in SCC4 cells transfected with various PLC-γ1 constructs. SCC4 cells were transfected with various PLC-γ1 constructs as indicated. Transfected cells were selected by 4-day incubation in 300 µM G418, and then treated with EGF (50 ng/ml) for 2 minutes. Cells were harvested and total cell lysates were isolated. PLC-γ1 in the total cell lysates was immunoprecipitated using an antibody against the C-terminus of PLC-γ1, and PLC-γ1 activity was assayed. The results are expressed as percentages of the values obtained with vector-transfected/vehicle-treated cells. Data are mean ± SD of triplicates within a single representative experiments, *P< 0.01 (highly significantly different from the vehicle treated cells). Results shown are representative of three independent experiments.