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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Insect Physiol. 2010 May 5;56(10):1414–1419. doi: 10.1016/j.jinsphys.2010.04.013

Figure 2.

Figure 2

Accumulation of eggshell-related transcripts in Anopheles gambiae ovaries. Total RNA samples extracted from dissected ovaries or carcasses (body without ovaries) were utilized as templates in RT-PCR reactions. Amplified products were resolved by electrophoresis in 1% agarose gels and stained by ethidium bromide. Two parallel reactions were performed for each target transcript, one with eggshell-transcript specific primers (top signal pair) and the second with primers that amplify a fragment of the An. gambiae S60 ribosomal protein transcript (bottom signal pair).