Table 3. Tm-values and thermodynamic data of duplex formation of ps self-complementary oligonucleotidesa.
| Tm(°C) | ΔTm/mod (°C) | ΔH° (kcal/mol) | ΔS° [cal/(mol K)] | ΔG°310 (kcal/mol) | ||
|---|---|---|---|---|---|---|
| 5′-d(iG-C-iG-C-iG-C)b | 18 | 33 | –34 | –88 | –6.2 | |
| 5′-d(iG-C-iG-C-iG-C) | ||||||
| 5′-d(4-C-4-C-4-C)b | 19 | 41 | +1.6 | –47 | –129 | –7.8 |
| 5′-d(4-C-4-C-4-C) | ||||||
| 5′-d(5-C-5-C-5-C) | 20 | 57 | +4.8 | –64 | –162 | –10.2 |
| 5′d(5-C-5-C-5-C) | ||||||
| 5′d(6-C-6-C-6-C) | 21 | 62 | +5.8 | –46 | –116 | –9.9 |
| 5′d(6-C-6-C-6-C) | ||||||
| 5′-d(c7iG-C-c7iG-C-c7iG-C)b,c | 22 | 22 | –2.2 | –40 | –112 | –5.3 |
| 5′-d(c7iG-C-c7iG-C-c7iG-C) |
aMeasured in 1 M NaCl, 0.1 M MgCl2, 60 mM sodium cacodylate buffer, pH 7.
bSee Seela et al. (23).
cd(c7iG) = 7-Deaza-2′-deoxyisoguanosine 4 = c7z8iGd = 8-Aza-7-deaza-2′-desoxyisoguanosine. 5 = Br7c7z8iGd = 7-Bromo-8-aza-7-deaza-2′-desoxyisoguanosine. 6 = I7c7z8iGd = 7-Iodo-8-aza-7-deaza-2′-desoxyisoguanosine.