Table 4. Tm-values and thermodynamic data of non self-complementary ps duplexesa,b.
| Tm (°C) | ΔTm/mod (°C) | ΔH° (kcal/mol) | ΔS° [cal/(mol K)] | ΔG°310 (kcal/mol) | ||
|---|---|---|---|---|---|---|
| 5′-d(TiCATAAiCTiG iGAT)c | 23 | 44 | –85 | –242 | –10.3 | |
| 5′-d(A GTATTGA C CTA) | 24 | |||||
| 5′-d(TiCATAAiCT4 4 AT) | 25 | 43 | –0.5 | –73 | –206 | –9.0 |
| 5′-d(AGTATTGA CC TA) | 24 | |||||
| 5′-d(TiCATAAiCT 5 5 AT) | 26 | 47 | +1.5 | –76 | –211 | –10.0 |
| 5′-d(AGTATTGA CC TA) | 24 | |||||
| 5′-d(TiCATAAiCT 6 6 AT) | 27 | 49 | +2.5 | –77 | –214 | –10.7 |
| 5′d(AGTATTGA CC TA) | 24 |
aIn 1 M NaCl, 0.1 M MgCl2, 60 mM sodium cacodylate, pH 7; oligonucleotide concentration: 5 µM (single strand).
bd(iC) = MeiCd = 5-Methyl-2′-deoxyisocytidine.
cSee Seela and Wei (22).