. 2003 Dec 15;31(24):7150–7158. doi: 10.1093/nar/gkg926

Table 5. T_m-values and thermodynamic data of non self-complementary ps duplexes of the sequence 5′-d(ATiCiCAXTTATXA)·5′-d(TAGGTYAATACT)^a,b.

X·Y		T_m (°C)	ΔT_m/mod (°C)	X·Y		T_m (°C)	ΔT_m/mod (°C)	X·Y		T_m (°C)	ΔT_m/mod (°C)
iG·C^c	28·29	39
4·C	30·29	39	±0	5·C	35·29	43	+4	6·C	36·29	44	+5
4·G	30·31	–	>–20	5·G	35·31	–	>–20	6·G	36·31	–	>–20
4·T	30·32	27	–12	5·T	35·32	29	–10	6·T	36·32	31	–7
4·A	30·33	28	–11	5·A	35·33	28	–11	6·A	36·33	28	–11
4·iC	30·34	26	–13	5·iC	35·34	35	–4	6·iC	36·34	36	–3

^aIn 1 M NaCl, 0.1 M MgCl₂, 60 mM sodium cacodylate, pH 7; oligonucleotide concentration: 5 µM (single strand).

^bd(iC) = ^MeiC_d = 5-Methyl-2′-deoxyisocytidine.

^cSee Seela and Wei (22).

Table 5. Tm-values and thermodynamic data of non self-complementary ps duplexes of the sequence 5′-d(ATiCiCAXTTATXA)·5′-d(TAGGTYAATACT)a,b.